Ecommerce Solutions for Growth of Your eBusiness ~ iPhone App ...

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AP IMPACT: CO2 emissions in US drop to 20-year low

FILE - in this June 25, 2012 file photo, a crew works on a drilling rig at a well site for shale based natural gas in Zelienople, Pa. In a surprising turnaround, the amount of carbon dioxide being released into the atmosphere in the U.S. has fallen dramatically to its lowest level in 20 years, and government officials say the biggest reason is that cheap and plentiful natural gas has led many power plant operators to switch from dirtier-burning coal. Many of the world's leading climate scientists didn't see the drop coming, in large part because it happened as a result of market forces rather than direct government action against carbon dioxide, a greenhouse gas that traps heat in the atmosphere. (AP Photo/Keith Srakocic)

FILE - in this June 25, 2012 file photo, a crew works on a drilling rig at a well site for shale based natural gas in Zelienople, Pa. In a surprising turnaround, the amount of carbon dioxide being released into the atmosphere in the U.S. has fallen dramatically to its lowest level in 20 years, and government officials say the biggest reason is that cheap and plentiful natural gas has led many power plant operators to switch from dirtier-burning coal. Many of the world's leading climate scientists didn't see the drop coming, in large part because it happened as a result of market forces rather than direct government action against carbon dioxide, a greenhouse gas that traps heat in the atmosphere. (AP Photo/Keith Srakocic)

Chart shows carbon dioxide emissions from burning coal

(AP) ? In a surprising turnaround, the amount of carbon dioxide being released into the atmosphere in the U.S. has fallen dramatically to its lowest level in 20 years, and government officials say the biggest reason is that cheap and plentiful natural gas has led many power plant operators to switch from dirtier-burning coal.

Many of the world's leading climate scientists didn't see the drop coming, in large part because it happened as a result of market forces rather than direct government action against carbon dioxide, a greenhouse gas that traps heat in the atmosphere.

Michael Mann, director of the Earth System Science Center at Penn State University, said the shift away from coal is reason for "cautious optimism" about potential ways to deal with climate change. He said it demonstrates that "ultimately people follow their wallets" on global warming.

"There's a very clear lesson here. What it shows is that if you make a cleaner energy source cheaper, you will displace dirtier sources," said Roger Pielke Jr., a climate expert at the University of Colorado.

In a little-noticed technical report, the U.S. Energy Information Agency, a part of the Energy Department, said this month that energy related U.S. CO2 emissions for the first four months of this year fell to about 1992 levels. Energy emissions make up about 98 percent of the total. The Associated Press contacted environmental experts, scientists and utility companies and learned that virtually everyone believes the shift could have major long-term implications for U.S. energy policy.

While conservation efforts, the lagging economy and greater use of renewable energy are factors in the CO2 decline, the drop-off is due mainly to low-priced natural gas, the agency said.

A frenzy of shale gas drilling in the Northeast's Marcellus Shale and in Texas, Arkansas and Louisiana has caused the wholesale price of natural gas to plummet from $7 or $8 per unit to about $3 over the past four years, making it cheaper to burn than coal for a given amount of energy produced. As a result, utilities are relying more than ever on gas-fired generating plants.

Both government and industry experts said the biggest surprise is how quickly the electric industry turned away from coal. In 2005, coal was used to produce about half of all the electricity generated in the U.S. The Energy Information Agency said that fell to 34 percent in March, the lowest level since it began keeping records nearly 40 years ago.

The question is whether the shift is just one bright spot in a big, gloomy picture, or a potentially larger trend.

Coal and energy use are still growing rapidly in other countries, particularly China, and CO2 levels globally are rising, not falling. Moreover, changes in the marketplace ? a boom in the economy, a fall in coal prices, a rise in natural gas ? could stall or even reverse the shift. For example, U.S. emissions fell in 2008 and 2009, then rose in 2010 before falling again last year.

Also, while natural gas burns cleaner than coal, it still emits some CO2. And drilling has its own environmental consequences, which are not yet fully understood.

"Natural gas is not a long-term solution to the CO2 problem," Pielke warned.

The International Energy Agency said the U.S. has cut carbon dioxide emissions more than any other country over the last six years. Total U.S. carbon emissions from energy consumption peaked at about 6 billion metric tons in 2007. Projections for this year are around 5.2 billion, and the 1990 figure was about 5 billion.

China's emissions were estimated to be about 9 billion tons in 2011, accounting for about 29 percent of the global total. The U.S. accounted for approximately 16 percent.

Mann called it "ironic" that the shift from coal to gas has helped bring the U.S. closer to meeting some of the greenhouse gas targets in the 1997 Kyoto treaty on global warming, which the United States never ratified. On the other hand, leaks of methane from natural gas wells could be pushing the U.S. over the Kyoto target for that gas.

Even with such questions, public health experts welcome the shift, since it is reducing air pollution.

"The trend is good. We like it. We are pleased that we're shifting away from one of the dirtiest sources to one that's much cleaner," said Janice Nolen, an American Lung Association spokeswoman. "It's been a real surprise to see this kind of shift. We certainly didn't predict it."

Power plants that burn coal produce more than 90 times as much sulfur dioxide, five times as much nitrogen oxide and twice as much carbon dioxide as those that run on natural gas, according to the Government Accountability Office, the investigative arm of Congress. Sulfur dioxide causes acid rain and nitrogen oxides lead to smog.

Bentek, an energy consulting firm in Colorado, said that sulfur dioxide emissions at larger power plants in 28 Eastern, Midwestern and Southern states fell 34 percent during the past two years, and nitrous oxide fell 16 percent. Natural gas has helped the power industry meet federal air pollution standards earlier than anticipated, Bentek said.

Last year the Environmental Protection Agency issued its first rules to limit CO2 emissions from power plants, but the standards don't take effect until 2014 and 2015. Experts had predicted that the rules might reduce emissions over the long term, but they didn't expect so many utilities to shift to gas so early. And they think price was the reason.

"A lot of our units are running much more gas than they ever have in the past," said Melissa McHenry, a spokeswoman for Ohio-based American Electric Power Co. "It really is a reflection of what's happened with shale gas."

"In the near term, all that you're going to build is a natural gas plant," she said. Still, she warned: "Natural gas has been very volatile historically. Whether shale gas has really changed that ? the jury is still out. I don't think we know yet."

Jason Hayes, a spokesman for the American Coal Council, based in Washington, predicted cheap gas won't last.

"Coal is going to be here for a long time. Our export markets are growing. Demand is going up around the world. Even if we decide not to use it, everybody else wants it," he said. Hayes also said the industry expects new coal-fired power plants will be built as pollution-control technology advances: "The industry will meet the challenge" of the EPA regulations.

The boom in gas production has come about largely because of hydraulic fracturing, or fracking. Large volumes of water, plus sand and chemicals, are injected to break shale rock apart and free the gas.

Environmentalists say that the fluids can pollute underground drinking water supplies and that methane leaks from drilling cause serious air pollution and also contribute to global warming. The industry and many government officials say the practice is safe when done properly. But there have been cases in which faulty wells did pollute water, and there is little reliable data about the scale of methane leakage.

"The Sierra Club has serious doubts about the net benefits of natural gas," said Deborah Nardone, director of the group's Beyond Natural Gas campaign.

"Without sufficient oversight and protections, we have no way of knowing how much dangerous pollution is being released into Americans' air and water by the gas industry. For those reason, our ultimate goal is to replace coal with clean energy and energy efficiency and as little natural gas as possible."

Wind supplied less than 3 percent of the nation's electricity in 2011 according to EIA data, and solar power was far less. Estimates for this year suggest that coal will account for about 37 percent of the nation's electricity, natural gas 30 percent, and nuclear about 19 percent.

Some worry that cheap gas could hurt renewable energy efforts.

"Installation of new renewable energy facilities has now all but dried up, unable to compete on a grid now flooded with a low-cost, high-energy fuel," two experts from Colorado's Renewable and Sustainable Energy Institute said in an essay posted this week on Environment360, a Yale University website.

How much further the shift from coal to natural gas can go is unclear. Bentek says that power companies plan to retire 175 coal-fired plants over the next five years. That could bring coal's CO2 emissions down to 1980 levels. However, the EIA predicts prices of natural gas will start to rise a bit next year, and then more about eight years from now.

Despite unanswered questions about the environmental effects of drilling, the gas boom "is actually one of a number of reasons for cautious optimism," Mann said. "There's a lot of doom and gloom out there. It is important to point out that there is still time" to address global warning.

___

Associated Press writers Seth Borenstein in Washington and Jonathan Fahey in New York contributed to this story.

___

Online:

U.S. Energy Information Agency: http://bit.ly/MRLOFR

Environment360: http://bit.ly/Qu8ebk

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/3d281c11a96b4ad082fe88aa0db04305/Article_2012-08-16-Gas%20Drilling-CO2%20Drop/id-4f3ad1f0f6ce407e86f7fa1942d69e8d

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Police work to ID body parts found in Mich. sewer

This photo provided by the Sterling Heights, Mich., Police department shows chunks of human flesh found by contractors clearing debris from the 9-foot, 6-inch-wide pipe Wednesday, Aug. 15, 2012 in Sterling Heights, Mich. Police investigating the discovery have not said if the parts came from one or more people and have given no hint as to the gender or identity of the body or bodies. Investigators say they don't believe the parts had been in the sewer very long. (AP Photos/Sterling Heights Police)

This photo provided by the Sterling Heights, Mich., Police department shows chunks of human flesh found by contractors clearing debris from the 9-foot, 6-inch-wide pipe Wednesday, Aug. 15, 2012 in Sterling Heights, Mich. Police investigating the discovery have not said if the parts came from one or more people and have given no hint as to the gender or identity of the body or bodies. Investigators say they don't believe the parts had been in the sewer very long. (AP Photos/Sterling Heights Police)

(AP) ? Authorities investigating the discovery of more than a dozen chunks of human flesh and body parts in a suburban Detroit sewer have released photos of tattoos featured on some of them.

Sterling Heights police Lt. Luke Riley says the Macomb County medical examiner's office has been studying the small pieces found by contractors clearing debris from the 9-foot, 6-inch-wide pipe Wednesday morning.

On Thursday, the department released photos of some of the pieces, which are marked with what appears to be part of a tattoo. Police have not said if the parts came from one or more people and have given no hint as to the gender or identity of the body or bodies.

Investigators say they don't believe the parts had been in the sewer very long.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/386c25518f464186bf7a2ac026580ce7/Article_2012-08-16-Body%20Parts-Sewer/id-ce99737530294716ac4115435ebf6c27

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League of Legends

League of Legends

Seven of the most skilled and infamous treasure hunters are assembled for a job. When they are betrayed by their employer and left for dead, they must decide whether to get even or get the treasure. This is an epic play-by-post action/adventure/mystery.

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Toshiba Satellite U845W-S410

By Brian Westover

People have been using laptops to enjoy movies on the go for years. With an extra-wide screen and sweet-sounding Harman/Kardon speakers, the Toshiba Satellite U845W-S410 is targeted to be a dream laptop for movie lovers. But the oddly shaped ultrabook and its stretched dimensions may be more gimmicky than glamour and glitz.

Design
The U845W-S410's standout feature is its extra-wide screen. That super-wide screen offers the same 21:9 aspect ratio seen in CinemaScope movies, and is designed to play widescreen movies full-screen, sans letterboxing. The result is a display with the unusual resolution of 1,792 by 768?essentially an 11-inch 1,366-by-768 display stretched an extra three inches wider. Measuring 0.83 by 14.5 by 7.9 inches (HWD), the Satellite U845W sits as wide as a 15-inch laptop, like the Apple MacBook Pro 15-inch (Late 2011) , but the screen only has the height of an 11-inch screen, sitting even with the Apple MacBook Air 11-inch (Thunderbolt) . Weighing 3.65 pounds, it's heavier than most 14-inch ultrabooks, but lighter than 15-inch laptops. The design, though stretched by the widescreen display, looks sharp, with tinted metal on the lid and chassis and a wide stripe of soft-touch texturing across the palmrest and the leading edge of the lid.

The extra-wide screen is an interesting idea, but it raises plenty of questions. What about every other type of video, like non-Cinemascope movies, TV shows, YouTube videos, Netflix and Hulu offerings, or the hundreds of games optimized for the traditional 16:9 aspect ratio? Anything that doesn't conform to these wide dimensions will be letterboxed anyway, but with 1.5-inch wide black bars on either side instead of on top and bottom. This is assuming you've got everything set the usual 1,366-by-768 resolution?anything set higher will look fine width-wise, but will still be cut off along the top. Watching a standard 4:3 image will look even smaller: reruns from the age of classic TV could be displayed in a small square surrounded by black bars above and to the sides. If you zoom in, the sides of the screen will still be empty. For a high resolution picture without the wonky dimensions, the Editors' Choice Asus Zenbook Prime UX32VD-DB71 boasts a 1,920-by-1,080 resolution display with IPS technology, and its Core i5-equipped sibling, the Asus Zenbook Prime UX31A-R5102F has that same display at a price comparable to the U845W.

While the U845W claims ultrabook status due to its thin profile and light weight, the reality is that it is too wide for convenient portability and too short for productive use. Despite the obvious focus on movies, you'll have to add your own external optical drive for DVD or Blu-ray playback. And with that extra width, the U845W won't fit into a case made for 14-inch laptops? because it's too long?while 15-inch laptop sleeves may fit it in one direction, but not the other.

On either side of the keyboard, you'll see perforated grilles covering two built-in Harman/Kardon speakers. These premium speakers are paired with SRS Premium Sound, so the audio quality is actually quite good, but the layout is puzzling. With such a long chassis, why isn't there a 10-key numeric pad? Speaking of the keyboard, the typing experience is fairly decent, with quiet chiclet keys and a backlight that illuminates in the dark without a lot of light leakage. The black chiclet keys have a semi-gloss finish, and there's a bit of flexing when typing in the center of the keyboard.

Features
Design aside, the Satellite U845W is well-outfitted with features. Three USB 3.0 ports provide speedy connectivity for drives and peripherals, including one port with sleep and charge for powering other devices like your smartphone or tablet. A card reader on the front of the laptop supports SD and Memory Stick formats (SD/SDHC, MS/Pro), a compact Ethernet jack lets you plug in when the 802.11n Wi-Fi won't cut it, and there are the usual headphone and microphone jacks. Bluetooth 4.0 supports wireless headsets, speakers, and plenty of other peripherals. An HDMI-out port lets you connect to an external display, but you can also stream wirelessly with WiDi, provided you have the necessary Netgear Push2TV adapter.

One disappointment is the lack of an optical drive. Though uncommon on 13-inch ultrabooks, slot-loading drives have made appearances in 14- and 15-inch models, like the Dell Inspiron 14z (Summer 2012) and the Samsung Series 5 Ultra (NP530U4B-A01US) , and the U845W seems a perfect fit, especially since so few streaming services offer the widescreen format this model is built around. The 500GB 5,400rpm hard drive and 32GB SSD cache are both the same capacity and speed as those seen in the HP Envy 4-1043cl and Lenovo IdeaPad U310.

Toshiba preloads the Satellite U845W with plenty of extras, from Microsoft Office 2010 Starter and Google Chrome to 30-day trials of both Norton Internet Security and Toshiba Online Backup, a service that starts at $4.95 per month for 25GB of cloud storage. Toshiba also includes a few other branded services, like Toshiba BookPlace and App Place for purchasing ebooks and apps, respectively. Toshiba covers the Satellite U845W-S410 with a standard one-year warranty on parts and labor.

Performance
Toshiba Satellite U845W-S410 The Satellite U845W is equipped with a third-generation 1.7GHz Intel Core i5-3317U processor, the same dual-core processor seen in the Lenovo IdeaPad U310 and the HP Envy 4, but offers better performance when paired with the Satellite U845w's 6GB of RAM. As a result, the Toshiba U845w scored 4,652 points in PCMark 7, ahead of both the Lenovo IdeaPad U310(3,570 points) and the HP Envy 4-1043cl (3,962 points). While CineBench scores were nearly identical (all hovering right around 2.40 points) since it's a pure processor speed test, the Satellite U845W's multimedia scores pulled ahead thanks to its extra RAM, completing Handbrake in 1 minute 58 seconds and Photoshop in 3 minutes 42 seconds.

Toshiba Satellite U845W-S410

Equipped only with Intel's integrated graphics solution (Intel HD Graphics 4000), the Toshiba Satellite U845W offered acceptable graphics performance, but it was met with complications due to the unusual size and resolution of its display. For example, while 3DMark 2007 ran just fine and scored 5,833 points at medium resolution and detail settings, the second run was completed at 1,280 by 768 rather than the 1,792-by-768 native resolution of the display. Consequently, the second score was weighed anecdotally, but isn't considered a standard benchmark score. Similarly, in gaming tests, Lost Planet 2 ran fine at 1,028-by-768 resolution and medium detail settings, producing 20 frames per second (fps), but when dialed up to native resolution, it ran at 9fps with a width of 1,796 pixels while chopping off the top half of the picture. Again, this score is considered anecdotal and won't be included as an official benchmark score. Crysis, meanwhile, was unable to run successfully at any resolution.

The Satellite U845W lasted 6 hours 42 minutes in MobileMark, which is decent but behind the HP Envy 4-1043cl (7:45) and HP Folio 13-1020us (8:47). While it won't get you through an entire workday, it provides enough longevity to enjoy a triple-feature, provided you aren't watching the super-extended versions of the Lord of the Rings trilogy.

If the Toshiba Satellite U845W-S410 were to be judged purely upon its performance and feature set, it would fare rather well, offering solid processing power and a healthy selection of ports and storage space. Unfortunately, the headlining feature of the laptop?the movie-friendly widescreen display?mars the overall user experience and distorts the dimensions of the laptop. For a superlative movie watching experience, the Editors' Choice Asus Zenbook Prime UX32VD-DB71 offers a 1080p display and excellent speakers, while the similarly-equipped Lenovo IdeaPad U310 and HP Envy 4-1043cl are both less cumbersome and less expensive.

BENCHMARK TEST RESULTS:

COMPARISON TABLE
Compare the Toshiba Satellite U845W-S410 with several other laptops side by side.

More laptop reviews:
??? Toshiba Satellite U845W-S410
??? Lenovo ThinkPad X1 Carbon
??? Apple MacBook Pro 15-inch (Mid 2012)
??? Samsung Series 5 NP535U3C-A01US
??? HP Envy Sleekbook 6z-1000
?? more

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Budget Travel | When choosing to use, note your own insurance

Use when selecting your own insurance, make sure that there is forward and cons with them. In most cases, the rental charge your credit card for repairs and you will be responsible for the coordination of the refund by your auto insurance.

The next time you rent a car, be they are confronted, with a question, provided by each, which rents out cars have, answer: want to rent car insurance? Even if you reject their coverage, be they often try, convince you to buy their insurance. If you were involved with the car in an accident, you must ensure that it will be repaired or replaced. If you opt out of the landlord insurance, you may or may not be covered by your auto insurance. If you maintain full coverage on your own vehicle, the chances are pretty good, that your insurance cover a rental. However they may have different coverages and deductibles, so will need to contact your insurance company or check their terms and conditions.

To some of these problems to avoid, you can rent car insurance. Their auto insurance premium is not affected. With the rental insurance, will take care everything for you the owner.

Car rental insurance is usually quite expensive on the other side. Their insurance company bases its prices on your records and statistics of the drivers with similar profiles to you. This is not the case with rental companies, give daily flat rates based on the probability that each driver can have an accident. To rent a car for long periods of time means that you could spend a large amount of money for insurance. For some numbers would be the daily rates much lower than paying a deductible of their company.

It will be important for you whether car is insurance, what you really want. If you are traveling to an unknown area with different laws, weather and more traffic, you are statistically at higher risk for an accident. You may be more likely rental insurance in a case like this to accept. In addition, a majority of the personal insurance will provide no coverage in other countries.

Some common credit card companies offer car rental losses limited protection. On the contrary, it covers not damage to property or personal injuries in the accident. Make sure you thoroughly with the terms and conditions of your credit card company be familiar, before you select this option.

Regardless of which option to choose, they all have their advantages and disadvantages. Based on your individual circumstances, you must decide which option is best for you: rental car insurance, personal insurance coverage or with your credit card.

Source: http://www.askearthman.org/when-choosing-to-use-note-your-own-insurance.html

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Arizona Real Estate Investment Success Series Tip #3 | Top Arizona ...

Phoenix, Scottsdale and Arizona Foreclosures

This entry was posted in Real Estate Investments and tagged business, choose, contrast, estate, generally, investment, money, office, real, reason, rental, residential. Bookmark the permalink.

Source: http://toparizonarealestatedealsblog.com/2012/08/arizona-real-estate-investment-success-series-tip-3-3-reasons-why-owning-a-commercial-property-may-make-you-more-money-in-arizona-real-estate-investment-459/

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Antisense approach promising for treatment of parasitic infections

ScienceDaily (Aug. 13, 2012) ? A targeted approach to treating toxoplasmosis, a parasitic disease, shows early promise in test-tube and animal studies, where it prevented the parasites from making selected proteins. When tested in newly infected mice, it reduced the number of viable parasites by more than 90 percent, researchers from the University of Chicago Medicine report in the Proceedings of the National Academy of Sciences.

This precisely focused therapy combines short strands of "antisense" nucleic acid-like material with a small peptide that can transport those strands through cell membranes and into parasites, where they disrupt genetic signals. A similar approach from a team at Yale, published in April, showed comparable promise as a treatment for the parasites that cause malaria.

"This was proof of concept," said study author Rima McLeod, MD, a toxoplasmosis expert and professor at the University of Chicago Medicine. "We were able to cross multiple membranes, to insert the antisense strands into parasites living within cells and prevent them from making several different proteins. We now think we can shut down any of this parasite's genes."

"This approach may even have a role in non-parasitic diseases," she added. It is currently being tested in drug-eluting stents, as a treatment for bacterial or viral infections, including Ebola, and in patients with Duchenne muscular dystrophy, where it can block production of the defective segment of a dysfunctional gene.

The parasite McLeod and colleagues focused on, Toxoplasma gondii, is "probably the most common parasitic infection in the world," she said. "It infects as many as one-third of all humans, about two billion people worldwide." T. gondii causes disease in those who have immature immune systems, particularly those infected in utero. It also can be devastating for those who are immune-compromised and when it causes eye disease.

"New medications are urgently needed," she said. The standard treatments can cause side effects and patients may become hypersensitive to them. There are no medicines that can eliminate certain latent stages of the parasite's life cycle. There is no vaccine for humans.

The new treatment consists of a phosphorodiamidate morpholine oligomer (PMO), a short DNA-like molecule that binds to messenger RNA, preventing it from being translated into protein. This is conjugated to a "transductive peptide," a small molecule that can ferry the PMO across cellular barriers. The combination is known as a PPMO. An earlier study from the McLeod lab showed that such transductive peptides could bring small molecules into the untreatable dormant phase of the parasite.

The researchers tested this system in infected cells in tissue culture and in live, recently infected mice. It was able to knock down production of several distinct proteins.

They first tested their PPMO against easily detectable biomarkers by inserting genes for yellow fluorescent protein and for luciferase, a protein responsible for fireflies' glow, into parasites. Then they exposed parasite-infested cells to low levels of a PPMO targeting one piece of those genes. This reduced yellow fluorescence or dimmed bioluminescence by 40 to 60 percent.

Next, they tested its ability to block production of an enzyme, dihydrofolate reductase (DHFR), that the parasite needs to make folate and to replicate. After 48 hours, DHFR production of intracellular parasites was markedly reduced. Antisense oligomers targeting another enzyme and factors that direct the activity of many genes, called "transcription factors," associated with parasite replication, "also were successful," the authors note, "reducing parasite replication."

When they tested the anti-DHFR PPMO in newly infected mice, the results were dramatic. Within 96 hours, treatment reduced the number of parasites by 83 percent to 97 percent, depending on the measurement technique.

This approach is "paradigm shifting," McLeod said. "It has the potential to abrogate any molecular target and underscores the variety of diseases for which such an approach might apply."

The technology still has a few problems, she said. These PPMOs have a narrow therapeutic index; they can be toxic at a little more than the lowest effective dose. And we have not yet developed a way to eradicate latent stages of T. gondii, which can lie dormant in retina or brain cells for years, but such studies are underway. The researchers hope to use this technique to awaken dormant parasites, and then use drugs or PPMOs that target replicating parasites to kill them or eliminate both active and dormant parasite stages by targeting their plant-like transcription factors and other proteins unique to the parasites that humans don't have.

Much of the credit for developing and testing this system should go to the study's lead author, McLeod said.

Bo-Shiun Lai, a 20-year-old rising fourth-year college student at the University of Chicago, stumbled into his research position in McLeod's laboratory but adapted quickly. "Although it was difficult at the outset," he said, "everyone in the lab helped me develop the project and hone my laboratory techniques along the way."

It paid off. He completed his senior thesis as a junior, with honors, and will be applying for PhD programs.

This study was funded by the National Institutes of Health and gifts from the Mann and Cornwell, Taub, Rooney-Alden, Engel, Pritzker, Harris, Zucker, Morel and Mussilami families. Additional authors include Kamal El Bissati, Ying Zhou, Ernest Mui and Alina Fomovska of the University of Chicago, and William Witola, now at Tuskeegee University in Alabama.

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The above story is reprinted from materials provided by University of Chicago Medical Center.

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Journal Reference:

  1. Bo-Shiun Lai, William H. Witola, Kamal El Bissati, Ying Zhou, Ernest Mui, Alina Fomovska, and Rima McLeod. Molecular target validation, antimicrobial delivery, and potential treatment of Toxoplasma gondii infections. Proceedings of the National Academy of Sciences, 2012; DOI: 10.1073/pnas.1208775109

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Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/most_popular/~3/AmR2kFXKx5w/120813155638.htm

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Unraveling intricate interactions, 1 molecule at a time

Monday, August 13, 2012

A team of researchers at Columbia Engineering, led by Applied Physics and Applied Mathematics Associate Professor Latha Venkataraman and in collaboration with Mark Hybertsen from the Center for Functional Nanomaterials at the U.S. Department of Energy's Brookhaven National Laboratory, has succeeded in performing the first quantitative characterization of van der Waals interactions at metal/organic interfaces at the single-molecule level.

In a study published online August 12 in the Advance Online Publication on Nature Materials's website , the team has shown the existence of two distinct binding regimes in gold-molecule-gold single-molecule junctions, using molecules containing nitrogen atoms at their extremities that are attracted to gold surfaces. While one binding mechanism is characterized by chemical interactions between the specific nitrogen and gold atoms, the other is dominated by van der Waals interactions between the molecule and the gold surface.

"A detailed understanding of van der Waals interactions is a key step towards design of organic electronic devices," says Sriharsha Aradhya, the study's lead author and a Ph.D. candidate working with Venkataraman. "Apart from the fundamental importance of these measurements, we are also excited about its applications. Understanding the effects of van der Waals interactions is tremendously important for creating and optimizing devices with organic building-blocks".

"Many proposals for future photovoltaic and flexible electronic devices are based on organic molecules because they are cost-effective," Venkataraman adds, "and scientists need to have a deeper understanding of these van der Waals interactions. Our work opens up the possibility of measuring and characterizing the strength of interaction between a variety of molecules and metallic surfaces a single-molecule at a time."

The forces of attraction between atoms and molecules come in different varieties and strengths, Aradhya explains. One of the most ubiquitous forms of attraction in nature is the van der Waals force. In contrast to specific interactions arising from bonding between atoms, van der Waals interactions represent non-specific interactions with subtler underpinnings. While originally intended to explain the apparent continuity between gaseous and liquid phases of matter, these forces have come to be recognized as an important aspect in answering such diverse questions as how does water boil inside a pressure cooker? How are geckos able to climb walls vertically? Or how can you control the organization of molecules for an organic LED smartphone display screen?

As devices like the latter become increasingly common, there has been a good deal of renewed interest in van der Waals interactions, known to lie at the heart of the structure and functionality in such devices. These interactions between organic molecules and metallic surfaces are central to a diverse range of phenomena such as catalysis of reactions, molecular electronic architectures, and molecular self-assembly in nature and engineered material. However, van der Waals interactions remain challenging to characterize directly at the fundamental, single-molecule level both in experiments and in theoretical calculations.

For this Columbia Engineering study, the researchers used their custom-built conducting atomic force microscope to make simultaneous measurements of force and conductance in single-molecule junctions. They combined their measurements with theoretical calculations and simulations, carried out in collaboration with Hybertsen at Brookhaven, in order to provide a unique quantitative measurement of the relative importance of specific and non-specific interactions at the single-molecule level, in a regime where both are comparable.

"In simple terms, conductance of the junction acts as a fingerprint of the structure," explains Aradhya. "At the same time, the measured force ? especially the force needed to rupture the junction ? can be used to deduce its mechanical properties."

While similar studies have been reported by a few research groups around the world, such precise studies have typically required the measurements to be carried out at very low temperatures and in high vacuum. Venkataraman's and Aradhya's experimental setup was optimized for very high sensitivities even at room temperature and ambient conditions. This allowed the team to perform thousands of individual single-molecule measurements, resulting in statistically robust results. The researchers then performed extensive density functional theory calculations to help them understand the mechanisms underlying their measurements.

"Taken together, this unique combination of our state-of-the-art experimental and theoretical efforts has resulted in this major progress in quantifying van der Waals interactions," Venkataraman says.

Future research, she adds, will include trying to control the interplay of van der Waals non-specific interactions with chemical modifications, in order to "achieve interesting functionality at the single-molecule level, which is an active area of research in our lab."

"We're very excited about this," she says, "as our efforts towards developing a reliable way to simultaneously measure force and conductance are yielding exciting new opportunities to relate structure, mechanics, and electronics at the single-molecule level."

As Hybertsen notes, "The development and validation of efficient theoretical models for the van der Waals interaction is still in its nascent stage. We expect that our work will also have an impact on this theoretical effort underway among many research groups around the globe to develop accurate treatment of van der Waals interactions."

The experiments were conceived by Aradhya and Venkataraman. The experimental tools and large dataset analysis techniques necessary for this study were developed by Aradhya, along with Michael Frei, a recent graduate of Venkataraman's lab. The modeling of the interactions and the calculations were conceived and executed by Hybertsen at Brookhaven.

###

Columbia University: http://www.columbia.edu

Thanks to Columbia University for this article.

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Source: http://www.labspaces.net/122503/Unraveling_intricate_interactions____molecule_at_a_time

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